Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Oct 23;6:607–616. doi: 10.1016/j.redox.2015.09.003

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 2 — Properties of targeted HyPer and SypHer variants in HEK 293 cells (A) Typical microscopy images of HEK293 cells stably expressing HyPer in the cytosol (cyto-HyPer) and mitochondrial matrix (mito-HyPer). (B) Effect of various extracellular H₂O₂ concentrations on the maximal cyto-HyPer ratio. The latter was calculated by dividing the maximal ratio value (R_max) by the pre-H₂O₂ ratio (R₀) for multiple cells (n=5) measured in two independent experiments. The experimental data was fitted using a sigmoidal (Logistic) fitting equation. (C) Effect of extracellular H₂O₂ application on the ratio signal (480 nm/420 nm) of cyto-HyPer and mito-HyPer (upper panel) and their individual 420 nm and 480 nm signals (lower panel). (D) Same as panel B but now for the effect of extracellular H₂O₂, the reducing agent dithiothreitol (DTT) and NH₄Cl on the signals of cyto-HyPer (left part) and cyto-SypHer (right part).