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. Author manuscript; available in PMC: 2016 Nov 4.
Published in final edited form as: Neuron. 2015 Nov 4;88(3):514–527. doi: 10.1016/j.neuron.2015.09.049

Table 1.

The penetrance of PLM morphological defects

short
posterior
neurite		 abnormal
branching		 dorsal
cell body		 overextended
anterior
neurite		 dorsal
anterior
neurite		 Number
of cells 1
Wild Type 0% 0% 5% 7% 0% 58
egl-5(u202) 93% 67% 53% 86% 15% 85
egl-5(tm4746) 94% 60% 44% 90% 11% 70
egl-5(u202);
mec-3p::egl-5(+) 		13% 18% 13% 21% 3% 62
egl-5(u202);
mec-3p::php-3(+) 		15% 67% 48% 30% 11% 54
egl-5(u202);
unc-86p::mec-3(+) 2 		90% 63% 50% 93% 9% 68
mec-3p::unc-62(+) 42% 19% 23% 62% 12% 52
mec-3p::ceh-20(+) 2% 4% 4% 6% 0% 48
mec-3p::unc-62(+);
mec-3p::ceh-20(+) 		52% 25% 32% 71% 11% 56
ceh-13(ok737);
mec-3p::unc-62(+) 3 		45% n.a. 20% 65% 10% 40
egl-5(u1034)
ceh-13(ok737) 4 		94% n.a. 38% 84% 9% 32
egl-5(u202)
unc-62(e644) 		96% 64% 48% 90% 14% 50

In egl-5 animals about 60% and 70% of PLM neurons showed the expression of TRN markers at 15 °C and 20 °C, respectively. Morphological characterization of PLM neurons from egl-5 animals were performed at 20 °C.

1

Percentages are based on the number of cells that express the TRN marker uIs115[mec-17p::RFP].

2

In egl-5 animals carrying the unc-86p::mec-3 transgene, 94% (68 out of 72) of the PLM neurons expressed the TRN marker mec-17p::RFP.

3

The arrest of ceh-13 animals at L1 stage prevented the examination of the branching of the PLM anterior neurites, which occurred at later developmental stages.

4

egl-5 ceh-13 double mutants were previously generated by inducing small deletion in egl-5 gene in ceh-13/+ heterozygotes using CRISPR/Cas9-mediated genome editing (Zheng et al., submitted).