Table 2. NA enzyme kinetics and antiviral susceptibility of European swine influenza viruses using MUNANA substrate.
The Michaelis–Menten constant (K m) and maximum velocity (V max) of substrate conversion were fitted to the Michaelis–Menten kinetics by non-linear regression. All values represent the mean (95% confidence interval).
| Virus | V max (µM min−1) | K m (µM) | IC50 (nM) | ||
| Oseltamivir | Zanamivir | Peramivir | |||
| H1avN2hu | 1364 (1257–1472) | 376.5 (272.6–480.4) | 0.10 (0.07–0.14) | 0.43 (0.25–0.75) | 0.14 (0.08–0.26) |
| H1pdmN2sw | 1845 (1655–2036) | 487 (320.5–653.6) | 0.29 (0.19–0.44) | 1.59 (0.85–2.98) | 0.48 (0.30–0.77) |
| H1N2 | 1495 (1219–1772) | 492 (191.7–792.4) | 0.47 (0.31–0.72) | 1.48 (0.86–2.56) | 0.66 (0.43–1.01) |
| H3N2 | 842.4 (743.9–940.9) | 281.1 (158.7–403.5) | 0.08 (0.05–0.12) | 0.14 (0.05–0.35) | 0.10 (0.07–0.14) |