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Fig. 3. Phylogenetic trees of the Simbu serogroup viruses. The trees were recreated using a maximum-likelihood method based on the general time reversible model (GTR) with five rate categories and assuming sites are evolutionary invariable, for the L gene (a), the GTR model with discrete gamma distribution for the M polyprotein gene (b) and the Tamura three-parameter model with discrete gamma distribution for the N gene (c). Bars, number of nucleotide substitutions per site. Positions with lower than 95 % site coverage were eliminated. Alignment and analysis were conducted in mega6 (Tamura et al., 2013) and final trees were created using FigTree v.1.4.2.