Fig. 2. Absence of detectable palmitoylation of NS4B purified from active HCV replication complexes. Huh7-Lunet cells overexpressing calnexin-HA (CANX^HA), or containing a JFH-1 wild-type replicon (NS4B^wt) or a JFH-1 replicon encoding HA-tagged NS4B (NA4B^HA), or transfected with expression vectors encoding HA-tagged apolipoprotein E (ApoE^HA) or the Myc-tagged Bet3 positive control protein (Bet3^Myc), were metabolically labelled with [³H]-palmitate followed by HA- or Myc-specific IP. Western blot analyses of unlabelled control samples that were processed in parallel are shown in (a). Conjugation of radioactive [³H]-palmitate to proteins was revealed using a β-imager and is depicted in (b). Molecular mass standards are indicated on the left of each blot.