Fig. 1. Membrane fusion efficiency of JEV and WNV VLPs as a function of temperature and lipid composition. (a, b) Fusion efficiency, measured as lipid mixing, of JEV (a) and WNV (b) VLPs is greater at 37 and 42 °C than at 25 °C. (c, d) Fusion efficiency of JEV (c) and WNV (d) VLPs with liposomes with compositions mimicking different cellular compartments. PM, plasma membrane; EEM, early endosomal membranes; MVB, multivesicular bodies; LEM, late endosomal membranes; BE, porcine brain extract lipids. Each value is the mean±standard deviation of triplicate measurements.