Fig. 3. Representative electropherograms from degenerate herpesvirus PCR. Open arrows indicate the described products. Size markers are shown in red with size (bp) indicated below. (a) Semi-nested POL assay. EBV control (250 copies) yields product of 238 bp dual-labelled in blue and green. (b) Semi-nested POL assay. HHV-6B control (iciHHV-6B DNA; 500 copies) yields product of 238 bp dual-labelled in blue and green. (c) Semi-nested POL assay. HCMV control (clinical isolate; 5000 copies) yields product of 313 bp dual-labelled in blue and green. The product >350 bp labelled in blue only is non-specific. (d) Semi-nested POL assay. Canine sample yields product of 200 bp dual-labelled in blue and green; sequencing revealed canine genomic sequence. Products >350 bp labelled in blue only are non-specific. (e) Gammaherpesvirus POL assay. Canine sample yields products dual-labelled in blue and green at ~200 and 310 bp; sequencing revealed canine genomic sequence. (f) gB assay. HHV-8 control (150 copies) yields product of ~500 bp labelled in blue and green.