Fig. 4. Analysis of canine samples using a gammaherpesvirus-specific PCR assay. Representative gel images. Positions of 100 and 200 bp markers are indicated; the specific product in this assay would be expected to be ~150 bp. M, marker; Nam, positive control (Namalwa); S18-20, canine samples. (a) Tumour DNA as template. No products are detected in sample, water or positive control lanes. (b) PCR products of semi-nested POL assay as template. A band of ~200 bp (arrow) is seen in the positive control lane. Sequencing confirmed that this was derived from EBV. No bands of this size are seen in sample or water lanes.
