Table 3. Primer sequences.

Primer	Sequence (5′→3′)*	Reference†
Canine γ-actin 5′	6-FAM-ACCACTGGTATTGTCATGGACTCTG	Gentilini et al. (2009)
Canine γ-actin 272 bp 3′	GCTCTTCTCCAGGGAGGACGA	Gentilini et al. (2009)
Canine γ-actin 720 bp 3′	TGGCTTTTAGCTCACGGCACC
POL1A (5′ outer)	HEX-GACTTTCCAAGTTTCTAYCCNAGYATHAT	Gallagher et al. (2002)
POL1B (5′ outer)	HEX-GACTTTCCAAGTTTCTAYCCNTCNATHAT	Gallagher et al. (2002)
POL2B (3′ outer and inner)	6-FAM-TTGATTAAGACGGAGTCNGTRTCNCCRTA	Jarrett et al. (2013)
POL3A (5′ inner)	HEX-GTTTGATGCCGACCTTAYGGNTTYACNGG	Gallagher et al. (2002)
POL3B (5′ inner)	HEX-GTTTGATGCCGACCTTAYGGNGTNACNGG	Gallagher et al. (2002)
2760s (gB 5′)	HEX-AAGATCAACCCCACNAGNGTNATG	Ehlers et al. (2008)
2761as (gB 3′)	6-FAM-GTGTAGTAGTTGTACTCCCTRAACATNGTYTC	Ehlers et al. (2008)
Huang 5′	GGGGTGGCCAACGGCCTCTTT	Huang et al. (2012)
Huang 3′	TMMTYCGTAGCTGACTCGGGTGA	Huang et al. (2012)
BamHI W 5′	CCCCTGGTATAAAGTGGTCCTG	Gallagher et al. (2003)
BamHI W probe	6-FAM-AGCTATTTCTGGTCGCATCAGAGCGC-TAMRA	Gallagher et al. (2003)
BamHI W 3′	CCCTCTTACATTTGTGTGGACTCC	Gallagher et al. (2003)

^*Dye labels are shown where appropriate. Clamp sequences in POL primers are shown in italics. Degenerate bases: Y = C or T; N = A, C, G or T; H = A, C or T; R = A or G; M = A or C.

^†References indicate source of previously described primers.