Fig. 3. β-Lactam sensitivity of a (p)ppGpp0 strain is unaffected by sugar. (a) Bacterial cell survival was determined after 18 h of treatment without (black bars) or with 30 μg carbenicillin ml− 1 alone (chequered bars) or in combination with 12 mM l-arabinose (white bars), 10 mM d-glucose (grey bars) or 10 mM d-mannitol (dotted bars) for WT, ΔrelA and ΔrelAΔspoT strains producing decreasing amounts of the (p)ppGpp alarmone. Data shown as mean ± sd (n ≥ 4). *p < 0.05. (b–d) Flow cytometry FSC versus SSC profiles of WT (b), ΔrelA (c) and ΔrelAΔspoT (d) strains after 18 h of treatment with 30 μg carbenicillin ml− 1 in combination with 10 mM d-glucose.
