Fig. 6. Small unambiguous RNAs mapping to the pilS6 locus in N. gonorrhoeae MS11. Small RNAs were isolated from N. gonorrhoeae strain MS11. RNA Seq analysis was employed to retrieve unambiguous transcripts that mapped to the pilS6 locus. Gonococcal transcripts that mapped sense to the pilS6 locus are shown as green, while anti-sense transcripts are shown as red. The pilS6 copies are shown in light blue (pilS6c3), purple (pilS6c2) and brown (pilS6c1), while the location of the minicassettes are shown in light grey (semi-variable) and dark grey (hyper-variable). The location of the experimentally verified pilS6c2 promoter is shown in yellow with the direction of ensuing transcripts shown with the faint grey arrow. Conserved regions in the pilS6 locus that share sequence identity to the pilS6c2 promoter are also shown in yellow with grey arrows indicating the direction of transcription. The experimentally verified anti-sense promoter is shown in orange with a grey arrow indicating the direction of transcription. The primer pairs utilized for qRT-PCR transcript detection from the pilS6c2 promoter are shown above.