Fig. 5. Alternatively activated BMDMs are more susceptible to cell death following infection with A/WSN/33, but do not support higher levels of virus replication. BMDMs from 129Sv/Ev and IFN-γR^{− / −} mice were untreated or activated with IFN-γ or IL-4 and infected with 10 p.f.u. A/WSN/33 per cell (WSN). Cell viability was measured by a CellTiter-Blue Viability Assay at various times after infection. (a) 129Sv/Ev BMDMs and (b) IFN-γR^{− / −} BMDMs. ***P < 0.001. (c) Virus titres in cell supernatants at 1 and 48 h p.i. were measured by titration on MDCK cells. Results are representative of three independent experiments.