Fig. 2. Oxidative stress in NiV-B-infected SAECs can be lowered using ROS scavengers. (a) Levels of lipid peroxidation product (F₂)-8-isoprostane quantified at the 48 h time point in supernatants of SAECs undergoing different drug treatments. The assay was performed with biological triplicates using 7.5 μM PDTC, 100 μM BHA or 2.5 μM Feb. ***P < 0.001 (Student's t-test). (b, c) Levels of lipid peroxidation product (F₂)-8-isoprostane quantified at the 48 h time point in supernatants of SAECs undergoing twofold dilution treatments with PDTC (b) and BHA (c). **P < 0.01 or ***P < 0.001 corresponds to a significant difference of (F₂)-8-isoprostane level between infected cells non-pre-treated or pre-treated with various PDTC (b) or BHA (c) concentrations (ANOVA and Bonferroni's multiple comparison test).