Fig. 3. Two doses of IVIG inhibit infiltration of Ly6C^high monocytes into the CNS of BALB/c mice infected with a high dose (10⁵ p.f.u.) of WNV. Mononuclear cells isolated from the brains of PBS- and IVIG-treated (25 mg) WNV-infected mice were analysed by flow cytometry for infiltrating cells at the indicated time points. Bar graphs depict percentage (L: left y-axis, depicted as bars) and number (#) (R: right y-axis, depicted as symbols) of (a) CD45^high infiltrating cells in the brain; (b) F4/80⁺ and (c) CD11c⁺ cells within the CD45^high infiltrating cells; and the pathogenic Ly6C^high subset within (d) F4/80⁺ macrophages or (e) CD11c⁺ cells. Data representative of two of three experiments; n = 3 mice per time point. (f) Percentage of the CD45^high infiltrating cells, Ly6C^high subset within F4/80⁺ macrophages or within CD11c⁺ Tip DCs isolated at day 21 p.i. from the brains of WNV-infected mice treated with a single (1×) or two (2×) doses of IVIG, as determined by flow cytometry. Data representative of two experiments; n = 3 mice. *P<0.05.