Fig. 5. IVIG suppresses CNS inflammation in BALB/c mice inoculated with a low dose (10⁴ p.f.u.) of WNV. Mononuclear cells isolated at the indicated time points from the brains of PBS-, IVIG (25 mg)- or WNV-IVIG (4 mg)-treated infected mice were analysed for CNS inflammation. Bar graphs depict percentage (L: left y-axis, depicted as bars) and number (#) (R: right y-axis, depicted as symbols) of CD45^high infiltrating cells (a), F4/80⁺ macrophages (b) and CD11c⁺ DCs (c) within the CD45^high infiltrates; and the pathogenic Ly6C^high subset within (d) F4/80⁺ monocytes and (e) CD11c⁺ DCs, as determined by flow cytometry. Data representative of three experiments; n = 3 mice per time point. *P<0.05, ***P<0.001).