Fig. 3. Cholangiocarcinoma in vivo expresses HCV entry factors (a) Flow cytometry data of HCV receptor expression in cholangiocarcinoma cells and control Huh-7 hepatoma cells. Expression levels are expressed as Mean Fluorescent Intensity (MFI) relative to species-specific control antibodies. (b) Confocal microscopic images of HCV receptors in permissive CC-LP-1 and Sk-ChA-1 cells. Scale bars represent 20 µm. (c) Claudin-1 expression in Huh-7 and CC-LP-1 cells analysed by Western blotting. (d) Real-time quantitative reverse-transcription PCR (qRT-PCR) analysis of claudin-1, -6 and -9 mRNA expression in Huh-7 and CC-LP-1 cells.