Fig. 5. HCVcc infection of cholangiocarcinoma cells. (a) Sk-ChA-1 cells were inoculated with HCVcc strain SA13/JFH (titre of 10⁶ IU ml⁻¹ based on Huh-7 cells) in the presence or absence of anti-HCV Ig (100 µg ml⁻¹), anti-CD81 antibody (clone 2s131, 10 µg ml⁻¹), protease inhibitor Telaprevir (1 µg ml⁻¹) and interferon-α (10 IU ml⁻¹). Data are presented as f.f.u. ml⁻¹ calculated as NS5A antigen-expressing cells. (b) Sk-ChA-1, but not CC-LP-1, cells support HCV SA13/JFH RNA replication at comparable levels to PHH. HCV RNA levels were normalized to Huh-7 cells. (c) Cholangiocarcinoma cells do not express miR-122 but control miR-210 was detected. (d) Sk-ChA-1 cells secrete low levels of ApoE, whereas we failed to detect ApoE from CC-LP-1 cells, where the dotted line indicates the ELISA cut-off point. N = 3 independent experiments.