Fig. 1. Effects of the siRNAs of interest on WT HIV-1 replication. HeLa cells in 24-well plates were sequentially transfected, first with the indicated siRNAs and 24 h later with pLAI. Then, 48 h later supernatant samples were harvested for (a) CA-p24 ELISA and (b) infectivity assay. The experiment was performed in triplicate and values represent mean±sd. LAI, Cells transfected with viral DNA alone; siCon, negative control siRNA; siDDX3, positive control; RLU, relative light units.
