Figure 7. Relating configurations in the EOT ensemble to the fully integrated IMP topology.

(A) Comparison of the average position of the slowest-flipping loop in the EOT ensemble to the average position of that same loop in the ensemble of fully integrated configurations. The average loop positions are presented in terms of the fraction of configurations for which each loop occupies the cytoplasm. The black dashed line indicates perfect correlation. (B) Fraction of N_cyto/C_cyto topologies for the CG trajectories that have reached full integration, excluding all trajectories for which at least one TMD is misintegrated, plotted as a function of time following the end of translation. The dashed lines indicate the fraction of N_cyto/C_cyto topologies for each mutant after all CG trajectories reach full integration, corresponding to the results from Figure 2.

DOI: http://dx.doi.org/10.7554/eLife.08697.017

Figure 7—figure supplement 1. Comparison of the average position of the slowest-flipping loop in the EOT ensemble to the average position of that same loop in the ensemble of fully integrated configurations.

For the K3, L85R, nEmrE, and nT28R¹ mutants, which have two slow-flipping loops, the axes of this plot are defined so as to account for the positions of both loops, as described in the Alternative definition of the slowest-flipping loop position for mutants with two slow-flipping loops section of the ‘Materials and methods’. The black dashed line indicates perfect correlation.

DOI: http://dx.doi.org/10.7554/eLife.08697.018