Figure 8. Predictions from the CG simulations in terms of changing the rate of ribosomal translation and introducing translocon mutations.

In each case, the figure presents the average position of the slowest-flipping loop in the EOT ensemble. The average loop position corresponds to the fraction of configurations for which that loop occupies the cytoplasm. The first column (dark gray) shows loop positions from the control simulation protocol. The remaining three columns show loop positions associated with the fourfold slowing of ribosomal translation (blue), associated with removal of the positive charge on the periplasmic opening of the translocon (red), and associated with removal of the negative charge on the cytoplasmic opening of the translocon (orange). The dashed boxes emphasize EmrE mutants that are predicted to exhibit dramatic inversions of the dominant topology upon translocon mutations.

DOI: http://dx.doi.org/10.7554/eLife.08697.019

Figure 8—figure supplement 1. Effect of changing the rate of ribosomal translation on loop positions.

In each case, the figure presents the average position of the slowest-flipping loop in the EOT ensemble. The average loop positions are presented in terms of the fraction of configurations for which each loop occupies the cytoplasm. Four different rates of ribosomal translation are presented: 24 residues/s (dark gray), 18 residues/s (blue), 12 residues/s (red), and 6 residues/s (orange).

DOI: http://dx.doi.org/10.7554/eLife.08697.020