Appendix table 2.
CG bead sequences for each of the 16 EmrE mutants and the cotranslationally-biased mutant
| Protein | L1 | TMD1 | L2 | TMD2 | L3 | TMD3 | L4 | TMD4 | L5 |
|---|---|---|---|---|---|---|---|---|---|
| EmrE | QQQQQ | VVVV | KQKQQ | LLLL | QQQQQ | LLLL | KQQQQ | LLLL | KQQQQ |
| EmrE(Ncyto) | QQQQQ | VVVV | KQQQQ | LLLL | QQQQQ | LLLL | QQQQQ | LLLL | KQQQK |
| EmrE(Nperi) | QQQQQ | VVVV | KKKQQ | LLLL | QQQQQ | LLLL | KKQQQ | LLLL | QQQQQ |
| K3 | KQQQQ | VVVV | KQKQQ | LLLL | QQQQQ | LLLL | KQQQQ | LLLL | KQQQQ |
| T28R | QQQQQ | VVVV | KKKQQ | LLLL | QQQQQ | LLLL | KQQQQ | LLLL | KQQQQ |
| A52K | QQQQQ | VVVV | KQKQQ | LLLL | QQKQQ | LLLL | KQQQQ | LLLL | KQQQQ |
| L85R | QQQQQ | VVVV | KQKQQ | LLLL | QQQQQ | LLLL | KKQQQ | LLLL | KQQQQ |
| R111 | QQQQQ | VVVV | KQKQQ | LLLL | QQQQQ | LLLL | KQQQQ | LLLL | KQQQK |
| nEmrE | QQQQQ | VVVV | QQQQQ | LLLL | QQQQQ | LLLL | QQQQQ | LLLL | QQQQQ |
| nK3 | KQQQQ | VVVV | QQQQQ | LLLL | QQQQQ | LLLL | QQQQQ | LLLL | QQQQQ |
| nT28R1 | QQQQQ | VVVV | KQQQQ | LLLL | QQQQQ | LLLL | QQQQQ | LLLL | QQQQQ |
| nT28R2 | QQQQQ | VVVV | KQKQQ | LLLL | QQQQQ | LLLL | QQQQQ | LLLL | QQQQQ |
| nT28R | QQQQQ | VVVV | KKKQQ | LLLL | QQQQQ | LLLL | QQQQQ | LLLL | QQQQQ |
| nA52K | QQQQQ | VVVV | QQQQQ | LLLL | QQKQQ | LLLL | QQQQQ | LLLL | QQQQQ |
| nL85R | QQQQQ | VVVV | QQQQQ | LLLL | QQQQQ | LLLL | KKQQQ | LLLL | QQQQQ |
| nR111 | QQQQQ | VVVV | QQQQQ | LLLL | QQQQQ | LLLL | QQQQQ | LLLL | KQQQQ |
| CB mutant | L1 | TMD1 | L2 | TMD2 | L3 | TMD3 | L4 | TMD4 | L5 |
|---|---|---|---|---|---|---|---|---|---|
| KQQQK | LLLL | QQQQQQQQQQ | LLLL | KQQQQQQQQK | LLLL | QQQQQQQQQQ | LLLL | KQQQQQQQQK |
Moderately hydrophobic (V), very hydrophobic (L), neutral-hydrophilic (Q) and charged (K) beads are colored as light gray, gray, black, and red respectively. Loops and TMDs are labeled.