Fig. 3. Y. pestis genes highly induced in the flea gut are involved in aggregation and biofilm phenotypes. (a) Bacterial autoaggregation was assessed by measuring the sedimentation of bacterial growth in static overnight LB/4 mM MgCl2/4 mM CaCl2 cultures (pH 5.5 and 7) incubated at room temperature. The mean ± sd of at least three independent replicates is indicated, grey bars denote ph7 and dotted bars denote pH5.5. (b) Image of biofilm formation in LB/4 mM MgCl2/4 mM CaCl2, pH 5.5 medium following vigorous overnight shaking in glass tubes at room temperature.
