Table 2. Stress response genes upregulated in Y. pestis ΔphoP during flea infection.
Criteria for significance: ≥ 2-fold difference, P ≤ 0.05 or ≥ 1.5-fold difference, P ≤ 0.001. Fold-change values in parentheses not significant based on P value. Genes in bold type are associated with an upstream consensus PhoP-binding site and are predicted or have been shown to be directly regulated by PhoP (Harari et al., 2010; Li et al., 2008; Perez et al., 2009).
Relative expression (fold-change; WT versus ΔphoP) during growth in: |
||||||
Gene ID | Gene name | Predicted function of gene product | Flea | LB (flow cell) | LB (exponential) | LB (stationary) |
Envelope stress/general stress response | ||||||
y0066 | cpxP | Periplasmic stress adaptor protein | − 5.5 | ( − 2.0) | ( − 2.0) | 4.5 |
y0137 | degQ | Endopeptidase; HtrA family protein quality control and stress response |
− 1.7 | 1.2 | − 1.2 | 1.0 |
y0295 | hslV | Endopeptidase; protein quality control | − 2.1 | − 1.2 | − 1.2 | 1.0 |
y0419 | rpoH | RNA polymerase factor sigma-32 | − 2.1 | − 1.1 | − 1.1 | − 1.3 |
y1265 | kdpA | Potassium-transport, osmotic stress | − 2.1 | 1.2 | − 1.0 | − 1.3 |
y1266 | kdpB | Potassium-transport, osmotic stress | − 3.1 | 1.1 | − 1.2 | (1.5) |
y1868 | htpX | Heat-shock protein | − 2.8 | − 1.1 | − 1.3 | 1.3 |
y3706 | dnaK | Heat-shock protein | − 3.0 | − 1.3 | 1.1 | 1.2 |
y4101 | ibpA | Heat-shock protein chaperone | − 5.2 | 1.3 | 1.2 | 1.3 |
y4102 | ibpB | Heat-shock protein | − 3.9 | (1.6) | − 1.1 | 1.4 |
Tellurite resistance genes | ||||||
y0555 | terZ | Tellurite resistance, stress response protein | − 3.7 | − 1.3 | − 1.2 | − 1.4 |
y0556 | terA | Tellurite resistance, stress response protein | − 2.5 | 1.0 | 1.0 | 1.4 |
y0557 | terB | Tellurite resistance, stress response protein | − 2.2 | 1.2 | − 1.2 | 1.0 |
y0558 | terC | Tellurite resistance, stress response protein | − 2.4 | 1.0 | − 1.1 | 1.1 |
TA | ||||||
Y1074 | – | relE/parE family addiction toxin module | − 3.8 | ( − 1.7) | ( − 2.0) | 1.0 |
Y1075 | – | phD family addiction antitoxin module | − 2.5 | ( − 1.6) | ( − 1.8) | 1.0 |
y3266 | mqsA | MqsA antitoxin | − 3.5 | ( − 1.8) | − 1.4 | 1.0 |
YPO0882* | mqsR | MqsR toxin | − 4.5 | ( − 2.6) | − 1.4 | 1.0 |
YPO1087* | – | HigB2 toxin | − 2.3 | − 1.2 | 1.0 | 1.1 |
YhcN family (DUF1471) multiple stress resistance | ||||||
y0666 | yhcN | Multiple stress resistance protein | − 9.3 | ( − 1.5) | − 1.3 | 1.1 |
y1667 | – | Multiple stress resistance protein | − 12.4 | ( − 1.9) | − 1.0 | − 1.2 |
* Gene not annotated in Y. pestis KIM (YPO number indicates Y. pestis CO92 homologue).