Fig. 1. Confocal images of E. coli stained with anti-Pal and vancomycin. (a) This pseudo-colour confocal image shows that only a small percentage of whole E. coli cells are stained with anti-Pal and Alexa Fluor 488 (green). Scale bar, 5 μm. (b) This merged pseudo-colour confocal image shows an E. coli cell stained with Pal and Alexa Fluor 647 (red), but not BODIPY FL vancomycin (green). Scale bar, 2.5 μm. (c) This pseudo-colour confocal image shows that BODIPY FL vancomycin (green) readily stains E. coli cells that were permeabilized by pretreatment with 0.1% Triton X-100.Scale bar, 2.5 μm.
