Fig. 4. Flow cytometric detection of Pal in a Pal deletion strain and WT E. coli. E. coli JC1292 cells (top) and JC7752 (Pal-deficient derivative) cells (bottom). The left panels are cells stained with Alexa Fluor 488 (Pal) and the right panels are stained with Alexa Fluor 647 (Pal). These data were representative of the three replicates that were collected for the experiment. The percentages of labeled bacteria (from total bacteria) are noted in the quadrants.