Table 1. Biotinylation of Pal in several E. coli strains.

Average biotinylated (B) and nonbiotinylated (NB) Pal populations, using Sulfo-NHS-SS-biotin and NHS-LC-LC-biotin with different E. coli strains, suggest that most of the Pal populations are nonbiotinylated.

E. coli strain	Biotin	NB (% population)	B (% population)
RS218	Sulfo-NHS-SS-biotin	74	26
RS218	NHS-LC-LC-biotin	74	26
XL1-Blue	NHS-LC-LC-biotin	72	28
Top10	NHS-LC-LC-biotin	69	31
JM109	NHS-LC-LC-biotin	72	28
JC1292	NHS-LC-LC-biotin	73	27