Fig. 2.

FDT morphometric analysis of mitochondria and synaptic puncta in SZ brains. FDT from dorsolateral PFC layer I of SZ (n = 6) compared to controls (CTRL; n = 2) suggests synaptic puncta of SZ subjects have lower density of (aggregated) mitochondria or abnormal mitochondrial morphology. a Fluorescence images from mitochondria and synaptic puncta immunohistochemistry co-localization used for FDT analysis. Left: Low-magnification image of TOMM40 immunofluorescence (i.e., mitochondria label). Scale bar = 10 μm. Right: High-magnification image of TOMM40 (mitochondria = red) and PSD-95 (synaptic puncta = green) immunofluorescence. Colors refer to the online version only. Scale bar = 1 μm. Arrows point to co-localization of both markers used for morphometric analysis between SZ and CTRL. b Mean and standard deviation of co-localized puncta (TOMM40+/PSD-95+) across all subjects suggests no difference between SZ and CTRL (i.e., the same number of co-localized puncta were observed in both groups). c Mean and standard deviation of co-localized puncta (TOMM40+/PSD-95+) across all subjects, binned according to TOMM40 fluorescence intensity and normalized to the total number of puncta counts per subject, demonstrates significant differences between SZ and CTRL and suggests SZ synapses have lower density of (aggregated) mitochondria or abnormal mitochondrial morphology. n.s. = Not significant, * p < 0.05, ** p < 0.01.