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Asymmetric synthesis of (R)-CHBE in aqueous/organic solvent (1:1) biphasic systems
| Strains | Enzyme activity (U/mg) | Yield (%) | ee (%) | ||
|---|---|---|---|---|---|
| GDH | CgCR | Aqueous/butyl acetate | Aqueous/toluene | ||
| E. coli/BmGDH-CgCR | 9.5 | 0.9 | 79.5 | 85.5 | >99 |
| E. coli/BmGDH-CgCR/pHACMC9 | 8.9 | 0.8 | 100 | 94.6 | >99 |
Reaction conditions: 0.5 g wet cells, 250 g/L glucose, 100 g/L of ethyl 4-chloro-3-oxobutanoate (COBE) in 5 mL potassium phosphate buffer (0.1 mol/L, pH 6.5) and 5 mL organic solvent, in 30 °C shaker for 2 h
