Figure 3. Inhibition of EAE by MOG_35-55-specific CD8⁺ T cells derived from MOG^−/− mice.

(A) Following the immunization of myelin oligodendrocyte glycoprotein (MOG)^−/− B6 mice with MOG_35-55, lymph node cells and splenocytes were harvested and carboxyfluorescein succinimidyl ester (CFSE) stained. Cells where then cultured in vitro with MOG_35-55 and 5 days later CFSE dilution measured. ∆ Proliferating fraction (PF) is the % of CFSE low CD8⁺ T cells in the MOG_35-55 stimulated condition minus the no antigen (background) condition. Data are representative of 3 independent experiments. (B) MOG-CD8⁺ T cells generated from MOG^−/− mice were injected IV into naive B6 mice and experimental autoimmune encephalomyelitis (EAE) was induced with MOG_35-55/complete Freund's adjuvant (CFA) immunization on day 0. Representative data of 2 experiments are shown, n = 5–7 per condition. *p < 0.05.