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. 2015 Nov 5;4:51. doi: 10.1186/s13742-015-0091-4

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© Wu et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Heterogeneity of gene expression in HeLa S3 single cells. a The mRNA molecular number in single cells and 10 pg RNA replicates. b The heat map of the FPKM values of extremely highly expressed genes (FPKM > 500 in bulk RNA) in single cells and 10 pg replicates. c Single-cell subpopulations identification based on cell cycle relative genes. The cells with underline are in G2/M phase. d Gene co-expression modules derived from 19 single cells based on RNA molecular number (modules are distinguished by colors). The detailed of each module stands for were shown on Additional file 4: Table S7. The weighted gene correlation network was constructed using the WCGNA R package [38]