Figure 2.
Effect of palmitic (PA) and oleic (OA) acids on markers of apoptosis. Trophoblast cells were treated for 24 h with 500 µM PA or OA. (A) Caspase3 and caspase 9 mRNA were measured by RT–PCR of the cell extracts. Results were normalized for beta-actin and expressed as fold changes versus control (CTL; cells treated with 2% BSA). Shown are means ± SEM, n = 3 placenta with triplicate wells/condition. 1.5 × 106/well primary cultured cells were plated in 12-well plate for each condition. There were no significant differences between control and fatty acid (FA) treated groups. (B) Lactate dehydrogenase (LDH) activities were measured in the culture medium to test the cytotoxicity resulting from 500 µM PA and OA by Cayman's LDH Cytotoxicity Assay Kit. Shown are means ± SEM, n = 6 placenta with duplicate wells/condition. There were no significant difference between CTL and FA treated groups.