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. 2015 Jul 22;30(9):2152–2159. doi: 10.1093/humrep/dev173

Figure 3.

Figure 3

Effect of fatty acids on toll-like receptor 4 (TLR4) and cytokine mRNA expression. Trophoblast cells were cultured with myristic acid (MA), palmitic acid (PA), stearic acid (SA), palmitoleic acid (POA), oleic acid (OA) or linoleic acid (LA), respectively, for 24 h at a concentration of 500 µM for MA, PA, SA, POA, OA and LA. Lipopolysaccharide (LPS) 100 ng/ml was used as positive control. Abundance of mRNA for toll-like receptor-4 (TLR4) (A), tumor necrosis factor-alpha (TNF-α) (B), interleukin-6 (IL-6) (C) and interleukin-8 (IL-8) (D). mRNA in trophoblast cells was measured by RT–PCR. Data were normalized for actin and calculated as fold changes versus Control (CTL; cells treated with 2% BSA for 24 h). Shown are means ± SEM, n = 14 independent experiments performed in triplicate, and 1.5 × 106/well primary cultured cells were plated in 12-well plate for each condition, *P < 0.05.