Skip to main content
. 2015 Jul 22;30(9):2152–2159. doi: 10.1093/humrep/dev173

Figure 4.

Figure 4

Effect of fatty acids on cytokine synthesis and release. Trophoblast cells were cultured with lipopolysaccharide (LPS), palmitic acid (PA), stearic acid (SA), palmitoleic acid (POA) and oleic acid (OA) for 24 h. The final concentrations in the medium were 100 ng/ml for LPS, 500 µM for PA, SA, POA and OA. Cells treated without or with 2% BSA for 24 h were used as negative controls (CTL). Levels of IL-6, IL-8 and TNF-α in the medium (B) and cell lysates (A) were measured by enzyme-linked immunosorbent assay . Data are shown as means ± SEM, n = 6 (A) and 3 (B), and 1.5 × 106/well primary cultured cells were plated in 12-well plate for each condition, *P < 0.05 versus CTL.