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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Int Immunopharmacol. 2015 May 21;27(2):224–231. doi: 10.1016/j.intimp.2015.05.018

Fig. 1. Effect of ASHMITM and extracts of its constituent herbs on TNF-α production.

Fig. 1

(A) RAW 264.7 cells were cultured with or without extracts for 24 hours, and then cultured in the presence of 1 μg/mL LPS for 24 hours. TNF-α levels in cell culture supernatants were determined by ELISA. Data are expressed as mean ± S.D. of three independent experiments. #, p<0.05 vs medium alone culture; **p<0.01 vs. LPS stimulated, but untreated (LPS alone) culture. (B) Percent inhibition was calculated in relation to LPS stimulated untreated cells.