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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Int Immunopharmacol. 2015 May 21;27(2):224–231. doi: 10.1016/j.intimp.2015.05.018

Fig. 2. Effect of 15 compounds isolated from G. lucidum on TNF-α production.

Fig. 2

(A). RAW 264.7 cells were pre-incubated with or without isolated G.lucidum triterpenoids (10 and 20μg/mL) for 24 hours, followed by addition of LPS (1 μg/mL) and culture for additional 24 hours. TNF-α levels in cell culture supernatants were measured by ELISA. Data are expressed as percent inhibition compared with LPS only group. (B). Concentration dependent GAC1 inhibition of TNF-α production by LPS-stimulated RAW 264.7 cells. (C). Cell viability of GAC1-treated and LPS-stimulated macrophages. Data are expressed as mean ± S.D. of 3–6 independent experiments. **p<0.01,*p<0.05 Vs LPS alone. (D). Cells were exposed to 0, 10, 20, 40 μg/mL GAC1 for 24 hours, followed by addition of LPS (1.0 μg/mL) and cultured for an additional 24 hours. Cells were then collected and subjected to Annexin V-FITC/PI staining and analysis by flow cytometry.