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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Int Immunopharmacol. 2015 May 21;27(2):224–231. doi: 10.1016/j.intimp.2015.05.018

Fig. 3. GAC1 inhibited TNF-α production when simultaneously co-incubated with LPS.

Fig. 3

(A). RAW 264.7 cells were treated by simultaneous co-incubation with various concentrations of GAC1 and LPS (1.0 μg/mL) for 24 hours. TNF-α levels in cell culture supernatants were determined by ELISA. Data are expressed as mean ± S.D. of 3–6 independent experiments. **p<0.01,*p<0.05 vs medium and LPS control groups. (B). RAW264.7 cells were treated with 0, 2.5, 5, 10, 20 and 40 μg/mL GAC1for 24 hours then subjected to MTT cell proliferation assay. Cell proliferation percentage was calculated by comparison with the medium alone group, which was set at 100%.