Fig. 4. GAC1 inhibited TNF-α production by PBMCs from asthma patients.
Purified PBMCs were treated with or without GAC1 (20 μg/mL) for 24 hours. LPS (2 μg/mL) was added and culture conditions maintained for additional 24 hours. Supernatants were harvested and TNF-α levels were determined by ELISA. (A). GAC1 (20 μg/mL) inhibition of TNF-α production by LPS-stimulated PBMCs from asthma patients. (B). PBMC viability after treatment with GAC1 (20 μg/mL). * p<0.05, paired t-test, (n=11).
