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. 2015 Aug 12;36(11):1372–1380. doi: 10.1093/carcin/bgv110

Figure 3.

Figure 3.

Epidermal skin thickness, expression of Ki-67 and induction of apoptosis in long-term SSL-treated SKH1 wild-type and caspase-7 KO mice. (A) Epidermal skin thickness after treatment with SSL. After H&E staining of skin samples, epidermal thickness was analyzed in five separate areas using EZ-C1 FreeViewer Gold software (v. 3.20; Nikon) and an average of three samples was calculated per group. Data are shown as mean values ± SD. The asterisk (*) indicates a significant (P < 0.05) increase in skin thickness. (B) Ki-67 expression was detected by IHC and significant changes are noted in the lower panel. (C) The expression of caspase-3, -7, p23 and survivin was detected by western blotting (upper panels). Significant changes in p23 and survivin are indicated (lower panels). (D) The induction of apoptosis after treatment with SSL detected by TUNEL assay. Apoptotic cells in skin tissue samples were detected (arrows) following the manufacturer’s instructions (Millipore Corporation, Billerica, MA). Similar results were obtained from three samples per group.