Table 1.
siRNA/primer sequence (5′ -> 3′) | Product size (bp) | |
---|---|---|
LKB1 siRNA | ||
siRNA_1 | GGACUGACGUGUAGAACAATT | |
siRNA_2 | GCUCUUACGGCAAGGUGAA | |
mRNA (cDNA) | ||
ABCB1 forward | GGAAGACATGACCAGGTATGC | 93 |
ABCB1 reverse | GCCAGGCACCAAAATGAAACC | |
TUBB3 forward | GCCTCTTCTCACAAGTACGTG | 130 |
TUBB3 reverse | CCCCACTCTGACCAAAGATGAA | |
β-Actin forward | CGTCACCAACTGGGACGACA | 130 |
β-Actin reverse | CTTCTCGCGGTTGGCCTTGG | |
Genomic copy number | ||
ABCB1 forward | TGCTGGTCCTGAAGTTGATCTGTGAAC | 248 |
ABCB1 reverse | ACATTAGGCAGTGACTCGATGAAAGGCA | |
GAPDH forward | CCCCACACACATGCACTTACC | 97 |
GAPDH reverse | CCTAGTCCCAGGGCTTTGATT | |
Bisulfate sequencing | ||
BS forward | GGAAGTTAGAATATTTTTTTTGGAAAT | 223 |
BS reverse | ACCTCTACTTCTTTAAACTTAAAAAAACC |