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. Author manuscript; available in PMC: 2016 Oct 28.
Published in final edited form as: Ind Eng Chem Res. 2015 May 11;54(42):10197–10205. doi: 10.1021/acs.iecr.5b01193

Figure 2.

Figure 2

Hydrogel-swelling behavior translated into improved protein loading and release capacities. (A) Loading effciency of anti-TNF-α antibody in P(MAA-g-EG) and P(MAA-co-NVP) microparticles (≤75 μm) formulated with TEGDMA, PEGDMA-400, and PEGDMA-1000 as cross-linkers. Hydrogel microparticles were incubated in an anti-TNF-α solution in PBS (pH 7.4), and 1 N HCl was added to the solution to lower the pH and collapse the microparticles. (B) Anti-TNF-α antibody release from P(MAA-g-EG) hydrogel microparticles synthesized with different cross-linkers: PEGDMA-1000, PEGDMA-400, and TEGDMA. Protein release from the microparticles was studied under dynamic pH conditions (from pH 3.0 to pH 7.4) to mimic the transit of the particles through the GI tract. Released protein was quantified using MicroBCA protein assay.