Table 1.

Cellular and humoral responses in the different vaccine groups

Cellular and humoral responses in the different vaccine groups
Vaccine groups	Cellular						Humoral
	IFNγ (%)		IL-4 (%)		Cytotoxicity (%)		IgG (%)
	Pre	Post	Pre	Post	Pre	Post	Pre	Post
Control	0.97 ± 0.28	0.46 ± 0.41	2.03 ± 0.47	16.26 ± 32.75	0.75 ± 0.05	0.82 ± 0.19	2.43 ± 1.66	15.26 ± 24.87
CCV	0.58 ± 0.23	1.08 ± 0.43*	6.12 ± 3.47	4.96 ± 4.92	0.351 ± 0.1*	0.61 ± 0.26	4.67 ± 3.23	41.32 ± 12.22*
CIV	1.19 ± 0.22	1 ± 0.33*	29.42 ± 17.49*	2.22 ± 0.43	0.41 ± 0.31*	0.68 ± 0.07	19.79 ± 14.13*	19.34 ± 9.83
FV	0.87 ± 0.16	0.5 ± 0.19	3.99 ± 0.96	1.5 ± 0.78	0.63 ± 0.08	0.74 ± 0.17	10.53 ± 7.35*	52.22 ± 20.35*

Notes: CCV, cancer cell vaccine group; CIV, co-incubation vaccine group; Control, control group; Cytotoxicity, in vitro capacity of isolated splenocytes to kill EO771 cells; FV, fusion vaccine group; IFNγ, IFNγ produced by CD4⁺ and CD8⁺ splenocytes stimulated with EO771 cell lysate (marker for CTL and Th1 activation); IgG, presence of IgG serum antibodies on EO771 cells; IL-4, IL-4 produced by CD4⁺ and CD8⁺ splenocytes stimulated with EO771 cell lysate (marker for Th2 activation); Post, 100 d post tumor inoculation or 102 d after the second vaccination; Pre, 2 d prior to tumor inoculation or 5 d after the second vaccination. Mean data with 1x standard deviation per group are presented. Significant results are indicated with asterisks.