Figure 7.

mTcd138 immunization of mice via i.p, i.m. or i.d. routes provides full protection against infection with a hypervirulent C. difficile strain. Mice were challenged with C. difficile UK6 spores (10⁶ /mouse) 14 days after the third immunization with mTcd138 or PBS. Kaplan-Meier survival plots (P = 0.0384 between PBS-immunized and the 3 mTcd138-immunized groups) (A), mean relative weight of all surviving mice (up to the day of death) (B) of different groups, and frequency of diarrhea (C) are illustrated. Data were presented as mean relative weight ± standard error.