Table 1.
Selected ICS peptides from H7N9 influenza and controls from circulating IAV strains or human proteins
| % Similarity with IAV |
Cross-conservation with Human Sequences |
||||||
|---|---|---|---|---|---|---|---|
| Peptide Description | Peptide Name | Peptide Sequence | Source Protein | A/California/7/2009 (H1N1) | A/Victoria/361/2011 (H3N2) | JanusMatrix Delta | # of Matches |
| Immunodominant HA peptides from circulating IAV strains | IAV-1 | PKYVKSTKLRLATG | HA | 100% | 85% | 6.70 | 10 |
| IAV-2 | PRYVKQSTLKLATG | HA | 85% | 100% | 8.45 | 13 | |
| IAV-3 | PRYVKQNTLKLATG | HA | — | 97% | 6.57 | 7 | |
| IAV-4 | PKYVKSNRLVLATG | HA | 89% | — | 9.37 | 11 | |
| H7N9 ICS peptides | H7N9-1 | RIDFHWLMLNPNDTVTFS | HA | — | — | 0.00 | 0 |
| H7N9-2a | YAEMKWLLSNTDNAAFPQ | HA | — | — | 6.37 | 8 | |
| H7N9-3 | KGILGFVFTLTVPSERGLQ | M1 | 100% | 100% | 6.77 | 10 | |
| H7N9-4 | QPEWFRNVLSIAPIMFSNK | PB1 | 97% | 99% | 11.50 | 14 | |
| H7N9-5 | GFTFKRTSGSSVKRE | PB2 | 93% | 93% | 12.05 | 17 | |
| H7N9-6 | RRDQKSLRGRSSTLGLDI | NS1 | 94% | 94% | 12.33 | 15 | |
| H7N9-7 | NYLLTWKQVLAELQDIE | PA | 96% | 97% | 13.00 | 14 | |
| H7N9-8 | DKLYERVKRQLRENAEED | HA | — | 83% | 13.22 | 24 | |
| H7N9-9 | AVKLYKKLKREMTFHGA | M1 | 97% | 95% | 13.68 | 35 | |
| H7N9-10 | AANIIGILHLILWILDRL | M2 | 96% | 100% | 15.36 | 16 | |
| H7N9-11 | SRKLLLIVQALRDNLEPG | PA | 100% | 98% | 16.99 | 51 | |
| H7N9-12 | QITFMQALQLLLEVE | NEP | 100% | 93% | 17.22 | 18 | |
| H7N9-13 | PRYVKQRSLLLATG | HA | — | 89% | 21.85 | 24 | |
| H7N9-14Ab | IVYWKQWLSLKNLTQ | PB1-F2 | — | — | 25.87 | 24 | |
| H7N9-14Bb | WKQWLSLKNLTQGSL | PB1-F2 | — | — | 26.05 | 25 | |
| Autologous peptides sharing identical TCR contact residues with selected H7N9 ICS peptides | 5-HUMAN-A | LSGLKRASASSLRSI | Rho GTPase-activating protein 42 | — | — | 27.97 | 36 |
| 5-HUMAN-B | RGILKRNSSSSSTDS | Synaptotagmin-like protein 2 | — | — | 37.89 | 38 | |
| 12-HUMAN | VFHFMQSLALLMSPV | ectopic p granules protein 5 homolog | — | — | 22.71 | 14 | |
| 14A-HUMAN | EEDLKQLLALKGSSY | mitochondrial NAD kinase 2 | — | — | 32.21 | 46 | |
| 14B-HUMAN | NLELLSLKRLTLTTS | Hyccin | — | — | 26.76 | 51 | |
Column 1: groups assigned to peptides based on their immunological characteristics. Column 2: peptide names. H7N9 ICS peptide names are ordered by JanusMatrix Delta. Human analog peptides are numbered according to their corresponding H7N9 peptide. Column 3: peptide sequence. Column 4: source protein of each peptide, either from IAV or the human proteome. Column 5: percentage of similarity with IAV. Similarity to circulating strains of IAV was determined by comparing each peptide to its corresponding sequence in either of the 2 IAV strains from the 2012/13 TIV. There was no conservation with Influenza B strain Wisconsin/1/2010 for any peptide. Any percentage that was lower than 80% was represented by ‘−’. Column 6: cross-conservation of each peptide with the human genome represented by JanusMatrix Delta and the number of matches found in the human database.aSimilar to avian H7.bSimilar to Trivalent Inactivated Influenza Vaccine and Live Attenuated Influenza Vaccine backbone strains.