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. 2015 Sep 18;94(37):e1508. doi: 10.1097/MD.0000000000001508

FIGURE 2.

FIGURE 2

A, Agarose gel electrophoresis of DNA against a 100 bp DNA ladder (Promega) following PCR amplification. Lanes 1 and 2, exon 2 of PRSS1 with a length of 310 bp; Lanes 3 and 4, exon 3 of PRSS1 with a length of 638 bp; Lanes 5 and 6, exon 3 of SPINK1 with a length of 691 bp. DNA sequences in affected and unaffected individuals. B, DNA sequence electropherogram of exon 2 of PRSS1 showing the wild-type sequence and the heterozygous A > C substitution (arrow) at nucleotide position c.86 that replaces asparagine with threonine at codon 29 (box) in the affected individual. C, DNA sequence electropherogram of intron 2 of SPINK1 showing the wild-type sequence at position c.88-352 (box) and the homozygous A > G polymorphism (arrow).