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. 2015 Jul 9;11(8):1961–1971. doi: 10.1080/21645515.2015.1066051

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Figure 1. — Construction and representative in vitro expression of BoNT/Hc/A, BoNT/Hc/B, and BoNT/Hc/EDNA vaccine constructs. (A) Schematic of BoNT/Hc/A, B, or E genes cloned into the pVAX1 mammalian expression vector. The CMV promoter, BoNT Hc gene(s), BGH poly A signal, kanamycin resistance gene, and pUC origin are shown. (B) Representative in vitro expression of the hemagglutinin-tagged BoNT Hc plasmids. Expression was confirmed using transfected RD cells and a HA-tagged antibody. An empty vector (pVAX) was used as a negative control. Results were analyzed with confocal imaging. Scale bar = 100 μm.