Table 2.

In vivo evaluation of protection against C. botulinum neurotoxin serotypes A, B, and E with antisera from mice vaccinated with the Hc monovalent vaccines

Group (n = 5)	Anti-seruma+ C. botulinum neurotoxin serotype used for challengeb	Survival against challenge with 102 LD50C. botulinum neurotoxin (%)c
1	Naïve + BoNT/A	0
2	BoNT/Hc/A + BoNT/A	100
3	Naïve + BoNT/B	0
4	BoNT/Hc/B + BoNT/B	100
5	Naïve + BoNT/E	0
6	BoNT/Hc/E + BoNT/E	100

^aAntisera were from naïve or vaccinated animals from the monovalent study.

^bAnimals were challenged with 200 μl of the respective toxin delivered by intraperitoneal injection.

^cAnimals were monitored for survival for up to 7 d post-challenge.