Figure 3.

Effects of miR-181a inhibition on leukemia cells susceptibility to NK cells. (A) NK-cell cytotoxicity of KG-1 and U-937 cells after miR inhibition with anti- miR-181a, anti-miR-146a, and anti-miR control. Cell viability 24 h post transfection was around 80%. NKL and NKD cells used as effectors (Representative curve of n = 5) (B) NK- cell mediated lysis of KG-1 and U-937 cells after transduction with anti-miR 181-a or empty vector transduced cells as negative control. NKL and NKD cells used as effectors (Representative curve of n = 5). (C) KG-1 and U-937 cells were transduced stably with Lenti-anti-mir-181a Lenti-anti-mir-control and cells were incubated with SLO (10 U/μL) alone or in combination with recombinant human GrB (5 ng/μL) for the indicated times. Apoptosis was assessed by flow cytometry using annexin 5 and 7AAD (Representative blot of n = 2).