HEV71 quantification. Changes in virus content during HEV71 propagation: showing virus quantity in: Vero cell lysate, direct culture supernatant, the top fraction fraction on top of virus pellet and the virus pellet, concentrated in high speed centrifuge at 35,000 rpm/minutes for 4 hours at 4°C, The mean viral contents on samples was measured on HEV-71-Ag quantification kit from (ABNOVA, Taiwan), (P = 0.0002).