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. 2015 Jun 30;11(8):1927–1935. doi: 10.1080/21645515.2015.1020265

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — Transfection efficiency and in vitro functional activity of bone marrow derived murine DC. (A) GFP expression in electroporated immature and mature (CpG treated) DC from BALB/c and C57BL/6 mice. Solid histograms are from untransfected cells and the numbers in the graphs represent the percentage of GFP⁺ DC. (B) Percentage of antigen-specific splenocytes stimulated with SIV Env gp160 DNA electroporated (D-iDC) or peptide pulsed (P-iDC) immature DC. Splenocytes cultured with DC without antigen loading or in the presence of Env peptide pools were in included as negative and positive controls respectively. The mean frequency (± SEM) of Env-specific IFN-γ⁺ T cells is shown from one representative out of 3 experiments.