Figure 4.

Env-specific cellular immune responses induced by prime-boost strategies. (A) Vaccination protocol listing the 4 vaccine platforms. C57BL/6 mice were immunized twice (week 0 and 4) and 2 weeks later splenocytes were collected and analyzed by flow cytometry for antigen-specific cellular responses. (B) Frequency (mean ± SEM) of Env-specific IFN-γ⁺ CD8⁺ T cells in vaccinated mice (one representative out of 3 experiments). p values (Mann-Whitney test) are given. (C) Frequency (mean ± SEM) of Env-specific CD8⁺ T cells with 1 function (IFN-γ⁺ or TNF-α⁺), 2 functions (IFN-γ⁺TNF-α⁺, IFN-γ⁺CD107a⁺, TNF-α⁺CD107a⁺) and 3 functions (IFN-γ⁺TNF-α⁺CD107a⁺) in CD8⁺ T cells. * p <0.05 (Anova): P-mDC VS DNA, P-mDC/DNA VS DNA. The general distribution of polyfunctional CD8⁺ T cells is shown in pie charts.